Figure 4.

Colocalization of renal biglycan and apoB. A: Shown are representative adjacent sections from a diabetic LDLR^−/− mouse fed the 0.12% diet and immunostained for biglycan and apoB (both red color product). The arrows indicate a glomerular foam cell. Scale bar = 25 μm. B: Shown is a representative section from a diabetic LDLR^−/− mouse fed the 0.12% diet immunostained for biglycan (green), apoB (red), nuclear DAPI stain (blue), and the merged image (colocalization yellow). Scale bar = 25 μm. C: Renal apoB content and renal biglycan content determined by densitometry were analyzed by linear regression. Solid symbols represent non-diabetic mice, open symbols represent diabetic mice. Squares represent mice on the 0% diet, circles represent mice on the 0.12% diet. Figure depicts four mice per group for all groups; r = 0.76, P < 0.001, and dashed lines represent 95% confidence intervals.