Table 1.
Primer Sequences and PCR Conditions for KISS1 Analyses
| Analysis | Sense primer | Antisense primer | Product size (bp) | Tanneal (°C) | PCR cycles (no.) |
|---|---|---|---|---|---|
| BS-SEQ | 5′-TGGAAGGGGAATAGTTTTATTAG-3′ | 5′-TACAACTAAAACTCCTTCRACC-3′ | 322 | 52 | 40 |
| MSP | 5′-CGGGTTGGAAGTTTTAGC-3′ | 5′-GCTTCGACAAACGAAAAAC-3′ | 125 | 54 | 37 |
| USP | 5′-TTTTGGGTTGGAAGTTTTAGT-3′ | 5′-ACTTCAACAAACAAAAAACAAC-3′ | 129 | 52 | 37 |
| RT-PCR | 5′-ACTCACTGGTTTCTTGGCAGC-3′ | 5′-ACCTTTTCTAATGGCTCCCCA-3′ | 183 | 60 | 28 |
BS-SEQ, bisulfite sequencing; MSP, methylation-specific PCR; Tanneal, annealing temperature; USP, unmethylated specific PCR.