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. 2011 Aug;179(2):754–765. doi: 10.1016/j.ajpath.2011.04.012

Figure 2.

Figure 2

Parietal cell loss in CCR7−/− mice is associated with decreased gastric Shh mRNA levels, hypochlorhydria, and increased levels of IL-1β. A: Immunoblotting for parietal H+/K+-ATPase α (HKα) and GAPDH in stomach tissue lysates of 11- to 23-month-old Wt (n = 8; lane 1–8) and CCR7−/− (n = 8; lane 9–16) mice. B: Quantitation of H+/K+-ATPase α protein expression normalized to GAPDH is shown. C: Quantitative PCR for Shh mRNA transcript in stomachs of 2-month-old Wt (n = 6) and CCR7−/− (n = 6) mice. Expression of Shh mRNA was normalized to GAPDH and is depicted as relative gastric Shh mRNA expression in CCR7−/− compared with Wt (set arbitrarily to 1) mice. D: Measurement of intragastric pH in 11- to 23-month-old Wt (n = 7) and CCR7−/− (n = 6) mice using pH indicator paper. E: Determination of IL-1β secretion from stomachs of 2-month-old Wt (n = 5) and CCR7−/− (n = 5) mice. Stomach-derived cells were cultured for 31 hours in RPMI 1640 medium without serum at 37°C. IL-1β release was measured in supernatants by cytokine bead array. Bars represent means ± SD (B and D) or SEM (C and E). *P < 0.05, **P < 0.01; Mann-Whitney U-test.