Figure 5.

Pea3 modulates the sensitivity of EC109 cells to doxorubicin treatment. A: PI-stained cells showing that the esophageal cancer cell line EC109 is highly resistant to doxorubicin treatment compared with immortalized NE1 cells. The results are representative of two independent experiments. B: Representative histograms of PI-stained cells under different conditions. C: A bar chart showing the percentage of cells in different phases of the cell cycle. D: Western blot analysis was used to detect apoptotic protein markers in cells that were treated with doxorubicin at different points during a 48-hour period. An MTT assay (E) and Western blot analysis (F) were used to detect apoptotic protein markers of EC109 cells expressing various levels of Pea3 that were treated with PI103 and doxorubicin, alone or in combination. *P < 0.05, **P < 0.01 compared with the shScr control.