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. 2011 Aug;179(2):964–979. doi: 10.1016/j.ajpath.2011.04.033

Figure 5.

Figure 5

Rotenone (Rot) exposure affects the levels of pro-oxidative and antioxidative enzymes. A: BV2 microglia were mock treated or treated with 30 nmol/L Rot for 24 hours, and intracellular expression levels of iNOS and COX-2 were determined by FACS (left). Rat primary microglia were mock treated or treated with 30 nmol/L Rot for 24 hours, after which iNOS levels were examined using Western blot analysis (right). B: The transcript levels of catalase, GPx-1, and GPx-2 (left) and MnSOD (bottom right) were determined by RT-PCR in primary microglia. The intracellular levels of MnSOD were determined by FACS analysis (top right). C: Primary microglial cells were mock treated or treated with 30 nmol/L Rot for the indicated times, and the total intracellular GSH content was measured using a kit from Cayman, according to the manufacturer's protocol. **P < 0.005 when compared with mock-treated control (CON) cells. GAPDH indicates glyceraldehyde-3-phosphate dehydrogenase; N.S., no significant difference.