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. 2011 Sep;179(3):1471–1482. doi: 10.1016/j.ajpath.2011.05.019

Figure 3.

Figure 3

The characteristics and tumorigenic activity of iC3-1. A: Morphology of iC3-1 cells (left, ×100 magnification) and confirmation of the forced expression of EGFP (right, ×100 magnification). B: RT-PCR analysis of CGB, HLA-G, and KRT. GAPDH was used as internal control. C: Western blot analysis of Phospho-p44/42 MAPK, p44/42 MAPK, Phospho-Akt and Akt. α-TUBULIN was used as a loading control. D: Measurement of the major axis of tumors derived from iC3-1 by subcutaneous injection into nude mice (mm). E: Survival curves for the nude mice injected with iC3-1 subcutaneously or intraperitoneally. F: iC3-1 cells also formed tumors exhibiting the characteristic two-cell pattern consisting of multinucleated syncytiotrophoblasts (dotted arrow) and mononuclear cytotrophoblasts (arrow) (left, ×200 magnification; right, ×600 magnification). G: Immunohistochemistry revealed that tumors formed by iC3-1 cells also expressed HLA-G (left, ×600 magnification) and cytokeratin (middle, ×600 magnification) hCG (right, ×600 magnification).