Figure 5.

Follistatin-overexpressing MPCs (FLST/OE MPCs) regenerated skeletal muscle more efficiently than WT MPCs, when transplanted into the GMs of mdx/SCID mice. A: Quantitation of engraftment in terms of the number of dystrophin-positive fibers regenerated by the FLST/OE and WT MPC populations. B: The overall mean ± SEM number of dystrophin-positive myofibers was significantly greater for the FLST/OE MPCs (592.8 ± 154.9; n = 7 FLST/OE MPC populations; four muscles per population) than for the WT MPC populations (195.6 ± 65.4; n = 5 WT MPC populations; four muscles per population; **P = 0.023, Student's t-test). C: Representative engraftments showed that the transplanted MPCs regenerated dystrophin-positive myofibers (red) within dystrophic muscle. FLST/OE MPCs produced more dystrophin-positive myofibers than did WT MPCs (*P < 0.05, **P < 0.01). Original magnification, ×200.