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. 2011 Sep;179(3):1360–1372. doi: 10.1016/j.ajpath.2011.05.048

Figure 6.

Figure 6

HO-1 expression in adult human astrocytes. Adult human glial cell cultures were prepared from surgical specimens as described in Materials and Methods following standard protocols. After 1 week in culture, individual microwell cultures were either left untreated (A, left) or treated with 3-HAA (A, right) or IL-1/IFN-γ and immunostained for GFAP and HO-1 expression, as described in Materials and Methods. Nuclei were stained with Hoechst (blue). The photographs depict HO-1–positive astrocytes (GFAP+) in adult glial cultures treated with 3-HAA. Scale bar = 10 μmol/L. B: Percentage of HO-1–positive astrocytes were quantified by counting double positive (HO-1+ and GFAP+) or single positive (GFAP+ alone) cells in culture, and this showed that 3-HAA treatment induced HO-1 in most astrocytes, whereas IL-1/IFN-γ had little effect (***P < 0.001). Results are representative of two independent experiments using different donor cells.