Skip to main content
. 2011 Aug;179(2):1030–1040. doi: 10.1016/j.ajpath.2011.04.026

Figure 4.

Figure 4

Leptin (Lep) induces nuclear translocation of GR and its phosphorylation (pGR). MCF-7 cells were untreated or treated with 1000 ng/mL of leptin for 15 minutes or pretreated with 10 μmol/L PD98059 (PD), AG490 (AG), or LY294002 (LY). A: Immunostaining of pGR and GR. No immunodetection was observed replacing the antibodies with horse serum [negative control (NC)]. B: Representative Western blotting for pGR on S211 and GR. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as loading control. The results are mean ± SE of three separate experiments for pGR in which the band intensities were evaluated in terms of optical density arbitrary units and expressed as the percentage of the control assumed to be 100%. *P < 0.05 versus untreated cells; **P < 0.05 versus leptin.