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. 2011 Aug;179(2):1030–1040. doi: 10.1016/j.ajpath.2011.04.026

Figure 6.

Figure 6

BRL antagonizes estrogen signaling induced by leptin (Lep). A: MCF-7 cells were transfected with XETL plasmid. CHO cells were cotransfected with XETL and HEGO plasmids (CHO/ERα). RLU, relative light unit. The results are mean ± SE of three independent experiments performed in triplicate. *P < 0.05 versus untreated cells; *P < 0.05 versus leptin. Cathepsin D, pS2, and cyclin D1 (B) and aromatase (C) mRNA expression in MCF-7 cells treated for 48 hours as indicated (left panel) and in xenografts (right panel). 36B4 mRNA levels were determined as control. NC indicates negative control. The results are mean ± SE of three separate experiments in which the band intensities were evaluated in terms of optical density arbitrary units and expressed as the percentage of the control assumed to be 100%. *P < 0.05 versus untreated cells; **P < 0.05 versus leptin.