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. 2011 Sep;179(3):1455–1470. doi: 10.1016/j.ajpath.2011.05.031

Figure 6.

Figure 6

Specific inhibition of neutrophil influx into PC-hi/diss primary tumors coordinately diminishes tumor angiogenesis and tumor cell intravasation. PC-hi/diss primary tumors were treated with 20 μg control IgG or IL-8/CXCL8-neutralizing antibody (anti IL-8) in three independent experiments. A: Immunohistochemical analysis of PC-hi/diss primary tumors. Sections from four to six individual tumors were stained with anti-human CD44 to differentiate human tumor cells (brown), anti-chicken MMP-9 antibody to visualize MMP-9–positive neutrophils (brown), and SNA to highlight blood vessels (brown). Original magnification, ×200. Scale bars = 50 μm. B: Tumor growth, neutrophil influx, tumor angiogenesis, and intravasation were quantified as described in Materials and Methods. Thirty and 22 tumor-bearing embryos were analyzed in control IgG- and anti IL-8–treated groups, respectively. Data are expressed as a percentage of IgG control and represent mean ± SEM. *P < 0.05, and ***P < 0.001, two-tailed Student's t-test.