Figure 2.

Comparison of transcript levels in attacked, nonattacked, and healthy control myofibers using TaqMan qPCR. Relative expression values were calculated using the 2^−ΔC_T method with cyclophilin as the endogenous control. Each symbol represents the mean of triplicate experiments performed on one sample. Five A_IBM samples (circles), five N_IBM samples (squares), and three H_CTRL samples (triangles) were included in the study. Colors correspond to individual patients (IBM-1, pink; IBM-2, green; IBM-3, gray; IBM-4, blue; IBM-5, yellow) and controls (C-1, white; C-2, gray; C-3, black), and medians are shown for each subset. Dashed line, detection limit. The following are shown: HLA-A (A), HLA-B (B), HLA-C (C), HLA-E (D), HLA-F (E), HLA-G (F), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (G). Data for HLA-E and HLA-G from A_IBM of patient 4 were out of range (>100) and are omitted. HLA-A and HLA-B were strongly up-regulated in both attacked and nonattacked sIBM myofibers. The nonclassic HLA (ie, HLA-E, HLA-F, and HLA-G) displayed a pattern similar to that of HLA-A and HLA-B. GAPDH was used as an additional endogenous control. As expected, its expression pattern largely mirrors that of cyclophilin in that it is unchanged in all samples.