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. 2011 Sep;179(3):1347–1359. doi: 10.1016/j.ajpath.2011.05.055

Figure 5.

Figure 5

IHC demonstrates differential IFNGR2 expression in patients with sIBM. IFNGR2 (Alexa 594 in red) and CD8 (Alexa 488 in green) were stained as described in Materials and Methods and visualized with confocal microscopy. Although some myofibers were strongly positive for IFNGR2 along their entire surface, others were only segmentally positive or negative. The strongly positive myofibers were often heavily attacked by CD8+ T cells. Herein, representative examples from one patient are shown. Four groups were defined according to IFNGR2 membrane positivity: group 1, >80% of the myofiber membrane positive for INFGR2; group 2, 50% to 80% of the myofiber membrane positive for INFGR2; group 3, 20% to 50% of the myofiber membrane positive for INFGR2; and group 4, <20% of the myofiber membrane positive for INFGR2. Images are organized clockwise from top left as follows: transmission, green channel (CD8 or respective control), red channel (IFNGR2 or respective control), and overlay. Scale bar = 50 μm.