Figure 1.

Ecto-ColXVII in the ECM of the migration track of cultured NHKs. A: IIF staining with the Ab HK139 revealed characteristic ringlike or arclike staining patterns that also contained numerous small dots around the migrating cell (arrows). B: The shed Ecto-ColXVII in the ECM was sensitive to collagenase digestion (the Ab HK139; bottom right), whereas laminin 332 (the Ab 6F12, bottom left) persisted. C: The shed Ecto-ColXVII in the ECM of cultured NHKs was detected by the Ab HK139 at 2 to 4 hours after seeding (arrows), and it remained stable for at least 8 hours. D: Thirty minutes after the shift of Ca²⁺ concentration in culture medium from 0.09 mmol/L to 1.2 mmol/L 4 hours after seeding, several NHKs detached, leaving ringlike deposition of Ecto-ColXVII in the ECM (arrows). Red, rhodamine phalloidin in A, C, and D and laminin 332 (the Ab 6F12) in B; blue, DAPI. Scale bars = 40 μm.