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. 2011 Aug;179(2):703–713. doi: 10.1016/j.ajpath.2011.04.030

Table 1.

Oligonucleotide Sequences of Trimming BAC Clone, Genotyping, Real-Time PCR Primers, and Northern Probes

Trimming BAC clone 5′-GGGTGCAAATTGCCCGGTGCCTTCTGTTTCCCAGGCAGCTCTGTG-3′
5′-CAGCTTACCTCTCAGGAATGCTACGTTTTGAACATCAAGAATGGAAA-3′
ACOX-amp
 Sense 5′-GCCATGGATATGTTCCAGAAGGTAGCTTGGTCTGACAGTTACCAATGC-3′
 Antisense 5′-GCTGCCATTGAGGCTTTTAACAAAGGTGGCACTTTTC-3′
Genotyping
 Neo
  Sense 5′-TATTCGGCTATGACTGGGCACA-3′
  Antisense 5′-GATGGATACTTTCTCGGCAGGA-3′
 mACOX1 exon
  Sense 5′-CCGCAAGCCATCCGACATTC-3′
  Antisense 5′-ATTCAGTGGGTCAGGCGACTGC-3′
 hACOX1BAC
  Sense 5′-ATTGCCCGGTGCCTTCTGTTTC-3′
  Antisense 5′-AGCCGGTGAGCGTGGGTCTC-3′
qPCR/Probe
 hACOX1
  Forward 5′-TCTGTCTGGGCCGCTGTCACTC-3′
  Reverse 5′-CCTAGGAGGCAGCCTCAGGACG-3′
 mACOX1
  Forward 5′-GCCAAGGCGACCTGAGTGAGC-3′
  Reverse 5′-ACCGCAAGCCATCCGACATTC-3′
 ATF6
  Forward 5′-CAGTTGCTCCATCTCCTCTCC-3′
  Reverse 5′-TGGGACACTGGCATTGGTTTG-3′
 XBP1
  Forward 5′-CCTGAGCCCGGAGGAGAA-3′
  Reverse 5′-CTCGAGCAGTCTGCGCTG-3′
 XBP1s
  Forward 5′-ACACGCTTGGGAATGGACAC-3′
  Reverse 5′-CCATGGGAAGATGTTCTGGG-3′
 ATF4
  Forward 5′-ACTATCTGGAGGTGGCCAAG-3′
  Reverse 5′-CATCCAACGTGGTCAAGAGC-3′
 GRP78
  Forward 5′-CGTGGAGATCATAGCCAACG-3′
  Reverse 5′-ATACGCCTCAGCAGTCTCCT-3′
 Trb3
  Forward 5′-CCCACAGGCACAGAGTACAC-3′
  Reverse 5′-CGTCCTCTCACAGTTGCTGA-3′
 CHOP
  Forward 5′-AGCCTGGTATGAGGATCTGC-3′
  Reverse 5′-CTCCTGCTCCTTCTCCTTCA-3′
 Gadd45α
  Forward 5′-CCAAGCTGCTCAACGTAGA-3′
  Reverse 5′-CCACTGATCCATGTAGCGAC-3′
 P8
  Forward 5′-ACCAAGAGAGAAGCTGCTGC-3′
  Reverse 5′-CTCCCTCTCCAGAACCTCACT-3′
 PPARγ
  Forward 5′-CCACAGTTGATTTCTCCAGCATTTC-3′
  Reverse 5′-CAGGTTCTACTTTGATCGCACTTTG-3′
 aP2
  Forward 5′-GAAGTGGGAGTGGGCTTTGC-3′
  Reverse 5′-TGTGGTCGACTTTCCATCCC-3′
 Bax
  Forward 5′-ACCAAGAAGCTGAGCGAGTG-3′
  Reverse 5′-CTCACGGAGGAAGTCCAGTG-3′
 Bcl2
  Forward 5′-TCTTCTCCTTCCAGCCTGAG-3′
  Reverse 5′-CCCACCGAACTCAAAGAAGG-3′
 18S
  Forward 5′-AAACGGCTACCACATCCAAG-3′
  Reverse 5′-CCTCCAATGGATCCTCGTTA-3′

BAC = bacterial artificial chromosome.