Table 1.
Trimming BAC clone | 5′-GGGTGCAAATTGCCCGGTGCCTTCTGTTTCCCAGGCAGCTCTGTG-3′ |
5′-CAGCTTACCTCTCAGGAATGCTACGTTTTGAACATCAAGAATGGAAA-3′ | |
ACOX-amp | |
Sense | 5′-GCCATGGATATGTTCCAGAAGGTAGCTTGGTCTGACAGTTACCAATGC-3′ |
Antisense | 5′-GCTGCCATTGAGGCTTTTAACAAAGGTGGCACTTTTC-3′ |
Genotyping | |
Neo | |
Sense | 5′-TATTCGGCTATGACTGGGCACA-3′ |
Antisense | 5′-GATGGATACTTTCTCGGCAGGA-3′ |
mACOX1 exon | |
Sense | 5′-CCGCAAGCCATCCGACATTC-3′ |
Antisense | 5′-ATTCAGTGGGTCAGGCGACTGC-3′ |
hACOX1BAC | |
Sense | 5′-ATTGCCCGGTGCCTTCTGTTTC-3′ |
Antisense | 5′-AGCCGGTGAGCGTGGGTCTC-3′ |
qPCR/Probe | |
hACOX1 | |
Forward | 5′-TCTGTCTGGGCCGCTGTCACTC-3′ |
Reverse | 5′-CCTAGGAGGCAGCCTCAGGACG-3′ |
mACOX1 | |
Forward | 5′-GCCAAGGCGACCTGAGTGAGC-3′ |
Reverse | 5′-ACCGCAAGCCATCCGACATTC-3′ |
ATF6 | |
Forward | 5′-CAGTTGCTCCATCTCCTCTCC-3′ |
Reverse | 5′-TGGGACACTGGCATTGGTTTG-3′ |
XBP1 | |
Forward | 5′-CCTGAGCCCGGAGGAGAA-3′ |
Reverse | 5′-CTCGAGCAGTCTGCGCTG-3′ |
XBP1s | |
Forward | 5′-ACACGCTTGGGAATGGACAC-3′ |
Reverse | 5′-CCATGGGAAGATGTTCTGGG-3′ |
ATF4 | |
Forward | 5′-ACTATCTGGAGGTGGCCAAG-3′ |
Reverse | 5′-CATCCAACGTGGTCAAGAGC-3′ |
GRP78 | |
Forward | 5′-CGTGGAGATCATAGCCAACG-3′ |
Reverse | 5′-ATACGCCTCAGCAGTCTCCT-3′ |
Trb3 | |
Forward | 5′-CCCACAGGCACAGAGTACAC-3′ |
Reverse | 5′-CGTCCTCTCACAGTTGCTGA-3′ |
CHOP | |
Forward | 5′-AGCCTGGTATGAGGATCTGC-3′ |
Reverse | 5′-CTCCTGCTCCTTCTCCTTCA-3′ |
Gadd45α | |
Forward | 5′-CCAAGCTGCTCAACGTAGA-3′ |
Reverse | 5′-CCACTGATCCATGTAGCGAC-3′ |
P8 | |
Forward | 5′-ACCAAGAGAGAAGCTGCTGC-3′ |
Reverse | 5′-CTCCCTCTCCAGAACCTCACT-3′ |
PPARγ | |
Forward | 5′-CCACAGTTGATTTCTCCAGCATTTC-3′ |
Reverse | 5′-CAGGTTCTACTTTGATCGCACTTTG-3′ |
aP2 | |
Forward | 5′-GAAGTGGGAGTGGGCTTTGC-3′ |
Reverse | 5′-TGTGGTCGACTTTCCATCCC-3′ |
Bax | |
Forward | 5′-ACCAAGAAGCTGAGCGAGTG-3′ |
Reverse | 5′-CTCACGGAGGAAGTCCAGTG-3′ |
Bcl2 | |
Forward | 5′-TCTTCTCCTTCCAGCCTGAG-3′ |
Reverse | 5′-CCCACCGAACTCAAAGAAGG-3′ |
18S | |
Forward | 5′-AAACGGCTACCACATCCAAG-3′ |
Reverse | 5′-CCTCCAATGGATCCTCGTTA-3′ |
BAC = bacterial artificial chromosome.