Table 2.
ChIP assay | |
P1 | |
Forward | 5′-CTGACGGAAGGAAGGTTGTT-3′ |
Reverse | 5′-TGGCTCATTTAGAGACACGC-3′ |
P2 | |
Forward | 5′-CAGCCAGACCAGAAATTGTC-3′ |
Reverse | 5′-GAGCCCATTGCACTTAGATG-3′ |
P3 | |
Forward | 5′-CTCAGACCTCAGGCCACTCT-3′ |
Reverse | 5′-CTTGGTGAGCTGGTGAGAAA-3′ |
EMSA | |
P1 | |
Sense | 5′-GGCACACCATCAAAGGGGAAAAGGTCAGAGGTTGGAAAACCTGAGCTCA-3′ |
Antisense | 5′-GAGACTCTGAGCTCAGGTTTTCCAACCTCTGACCTTTTCCCCTTTGAT-3′ |
P2 | |
Sense | 5′-GGATATGTCCCTGAGAGTTGCAGCTGACCCACAGCCTGCTGTGAGGCCACT-3′ |
Antisense | 5′-GGAATCAGTGGCCTCACAGCAGGCTGTGGGTCAGCTGCAACTCTCAGGGA-3′ |
P3 | |
Sense | 5′-GAGTAAAATTGCAACATTCCAAACTCAGACCTCAGGCCACTCTACACTTGGT-3′ |
Antisense | 5′-GGTTGAACCAAGTGTAGAGTGGCCTGAGGTCTGAGTTTGGAATGTTGCAATT-3′ |
ChIP = chromatin immunoprecipitation; EMSA = electrophoretic mobility shift assay.