Fig. 3.

(A) Levels of oxidatively damaged proteins were determined in the bladder of four age-matched control (AMC), four streptozotocin-diabetic and four insulin-treated) were detected by Oxyblot. A control reaction (‘con’) was run in which proteins were prepared in the absence of dinitrophenylhydrazine derivatizing agents and appear blank relative to reactions where the derivitization reagent was present (‘exp’). A representative blot of two animals from each of these groups is shown. (B) Nitrosylation of proteins in four AMC, diabetic and insulin-treated diabetic animals. A representative blot is shown for three animals from each group.