Figure 1.

GLTSCR2 is up-regulated and mobilized to the nucleoplasm in response to genotoxic stresses. A: SK-Hep-1 cells were exposed to UV (10 J/m²) (left) or irradiated with gamma rays (2 Gy) (right) and cultured under normal conditions for the indicated durations or treated with NCS (50 ng/mL) for varying durations as indicated (middle). Protein lysates were subjected to Western blots for the relative quantitative evaluation of GLTSCR2, phospho-ATM, ATM, phospho-H2AX, and H2AX. Tubulin was used as the loading control. B: Cells were untreated or treated as in panel A and fixed after the indicated times. Then, cells were co-immunostained with anti-GLTSCR2 and antinucleolin antibodies and viewed after nuclear staining with DAPI under the differential interference contrast–equipped inverted confocal microscope. Representative images are shown.