Table 2.
Antibodies and Corresponding Conditions for IHC
| Antibody | Clone | Supplier⁎ | Protocol | Cellular expression | Control tissue |
|---|---|---|---|---|---|
| GRP 94 | sc-1794 (C-19) | SCB | 1/2000† | Endoplasmic reticulum | Breast carcinoma |
| TRAF2 | SM7106P (clon 33A1293; 205–222 aa) | Acris | 1/100 O/N† | Cytoplasm | Breast carcinoma |
| FN14 | sc-27143 (C-13) | SCB | 1/3000† | Membrane | Kidney, heart |
| INHA | MCA951ST (R1) | AbD S | 1/50† | Cytoplasm | Testis |
| TOP1 | ab3825 (401–600 aa) | Abcam | 1/100‡ | Nuclei, cytoplasm | Colorectal tumor |
| VAV2 | sc-20803 (H-200) | SCB | 1/1000† | Cytoplasm | Pancreas |
| GFAP | Z0334 | Dako | 1/8000† | Cytoplasm | Brain (astrocytes) |
| TEM 8 | ab21270 | Abcam | 1/2000† | Cytoplasm, membrane | Brain tumor endothelium |
| ARFGAP | SP1402P | Acris | 1/1000† | Cytoplasm | Testis |
| EIF3s8 | ab19359 (N-terminal 1–50 aa) | Abcam | 1/1000 O/N† | Cytoplasm | Kidney |
| BAT 8 | G-6919 | Sigma | 1/250† | Cytoplasm | Lymph node |
O/N, antibody is incubated overnight.
⁎
Suppliers: Abcam, Cambridge, UK; AbD S, AbD Serotec, MorphoSys UK, Oxford, UK; Acris, Acris Antibodies, Herford, Germany; SCB, Santa Cruz Biotechnology, Santa Cruz, CA; Sigma, Sigma-Aldrich, St. Louis, MO.
†
Retrieved in Na-citrate buffer.
‡
Retrieved in Tris/EDTA.