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AK23 IgM recognizes the same Dsg3 epitope as AK23 IgG. A: The binding of AK23 IgM in a mouse Dsg3 ELISA was significantly blocked by AK23 IgG, but not by AK9 IgG or AK18 IgG. IgG(+), with AK IgG pretreatment; IgG(−), without AK IgG pretreatment. B: AK23 IgM reactivity was tested by mouse Dsg3 ELISA performed with and without EDTA (5 mmol/L) pretreatment.
