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. Author manuscript; available in PMC: 2012 Sep 2.
Published in final edited form as: J Mol Biol. 2011 Jun 22;411(5):999–1016. doi: 10.1016/j.jmb.2011.06.032

Fig. 2. KSHV Pr-DD2 titration.

Fig. 2

(a) The distribution of isoleucine δ1-methyl groups on a full length KSHV Pr monomer structure, based upon 2PBK. The view is a 90 degree rotation about the horizontal axis, relative to that in Figure 1b. Isoleucines separate into five zones with respect to distance from Trp109 as indicated by colored balls. The 13C-1H HSQC titration spectra of the KSHV Pr M197D (b) and Δ196 (c) constructs with DD2, focusing on the isoleucine δ1-methyl region. The spectral overlays display apo (black) and > 5 molar equivalents DD2 (red). Ile44 (solid blue box), Ile105 (dotted blue box), and Ile71 (solid black box) are used as the binding probes. (d) The binding curves (M197D, solid black; Δ196, dashed red) represent the average apparent Kd values calculated for the three Ile probes, and are summarized as a bar chart (e). HSQC titration spectra and binding curves for the M197D-I201V construct are displayed in Figure S4.