Skip to main content
. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Hypertension. 2010 Aug 16;56(4):705–712. doi: 10.1161/HYPERTENSIONAHA.110.154880

Figure 2. Increased superoxide production in IEX-1-deficient vasculature.

Figure 2

Dihydroethidium fluorescence intensity was greater in VSMCs in aorta of IEX-1 KO mice (G) relative to WT counterparts (A), but the intensity was comparable in the endothelium of the two strains of mice (F and L). The fluorescence intensity in IEX-1 KO VSMCs was diminished to a WT level by pretreatment with 1 µM rotenone (B and H) or 10 µM MnTBAP (C and I), but not with apocynin (D, E, J, and K) for 45 min. On representative result of three experiments performed with similar results. Lucigenin chemiluminescence was also greater in aortic rings obtained from IEX-1 KO mice, but it was diminished significantly after treatment with 1 µM rotenone (Rot) or 10 µM MnTBAP, but not with 100 or 500 apocynin (Apo) for 30 min (M). Data are expressed as mean ± SEM of scintillation counts per min/mg of dried tissue; n = 6. ** p<0.01 and ***p<0.001 in the presence or absence of the indicated anti-oxidant or IEX-1. Magnification: 40×. Bar = 25 µM