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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Hypertension. 2010 Aug 16;56(4):705–712. doi: 10.1161/HYPERTENSIONAHA.110.154880

Figure 3. An increase in mitochondrial superoxide production in IEX-1-deficient vasculature and cultured VSMCs.

Figure 3

Increased MitoSox fluorescence intensity was detected in vasculature in the absence compared to presence of IEX-1, but it was diminished to a WT level by pretreatment of the aortic rings ex-vivo with MitoQ 10 (100 nM) for 2 hrs (A). Shown in B are representative confocal microscopy images demonstrating an increase in MitoSOX Red fluorescence in cultured VSMCs isolated from the aorta of IEX-1 KO. The MitoSOX Red fluorescence was colocalized with MitoTracker Green as manifested by yellow fluorescence around the nuclei in merged images on the right panel. Magnification: 40×. Bar = 50 µM