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. 2011 Aug;179(2):1041–1049. doi: 10.1016/j.ajpath.2011.04.027

Figure 2.

Figure 2

Behavior of podoplanin-overexpressing mouse Dunn OS cells. A: Overexpression of human podoplanin in stably transfected Dunn cells. Cell lysates from the Dunn/PDPN stable transfectants (PDPN-11, -14, and -17) and mock cells were subjected to Western blot analysis with an anti-podoplanin antibody. B: Platelet aggregation–inducing ability of the Dunn/PDPN stable transfectants (PDPN-11, -14, and -17) was monitored using a whole-blood aggregometer following incubation with mouse whole blood as described in Materials and Methods. The aggregation reached a plateau within 20 minutes with the following aggregation amplitude: Mock: 2 ohms, PDPN-11, 6 ohms; PDPN-14, 8 ohms; PDPN-17, 40 ohms. Growth of the transfectants in monolayer culture using MTT assay (C) or in three-dimensional spheroid culture (D). Data are means ± SEM of triplicate determinations.