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. 2011 Aug;179(2):1041–1049. doi: 10.1016/j.ajpath.2011.04.027

Figure 5.

Figure 5

Podoplanin expression in human OS. Immunohistochemistry of podoplanin in normal human bone (A) and in human OS tissue (B–D). A: Intense podoplanin labeling of osteoid osteocytes (immature osteocytes) and canaliculi in normal bone. Mature osteoblasts (asterisk) occasionally showed membrane staining, and mature osteocytes expressed podoplanin very weakly (arrow). B: Osteoblastic tumor cells in a more mature OS undergoing “normalization,” also showing osteocytes embedded in irregularly shaped bone matrix. Osteocytes and osteocyte processes are clearly strongly positive for podoplanin (arrows). The insets show higher magnification of areas of interest. C: Undifferentiated OS with little extracellular matrix production. Pleomorphic and rounded tumor cells with rich cytoplasm showed intense membranous localization of podoplanin; D: Active osteoblastic component in an OS showing the typical malignant osteoid formation. Plump, cuboidal, osteoblastic tumor cells producing bone matrix (arrows) showed positive membrane staining for podoplanin, and occasionally showed antibody-labeled immature processes. E: Chondroblastic OS showing strong membranous staining for podoplanin. F: Fibroblastic OS with focal membranous immunoreactivity for podoplanin. G and H, I and J: Immunostaining of the same tumors shown in C and D, respectively, for CD44 (G and I) and pERM (H and J) showing membranous immunoreactivity of both CD44 and pERM. b, bone. Scale bars: 20 μm (A); 100 μm (B–F); 50 μm (G–J).