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. 2011 Sep;179(3):1385–1393. doi: 10.1016/j.ajpath.2011.05.018

Figure 4.

Figure 4

β-hCG expression results in transformation of immortalized ovarian surface epithelial cells. A and C: Growth rate of subcutaneous xenografts in nude mice inoculated with T29 β-hCG and T80 β-hCG cells, respectively: 13 of 24 injections in 10 mice grew tumors using T29 β-hCG cells, and 6 of 14 injections in 7 mice using T80 β-hCG cells, compared with an incidence of 0 tumors in 4 mice injected with T29 or T80 parental control cells, after 13 weeks of observation (mean ± SD). *P < 0.01. B and D: H&E staining of subcutaneous xenograft tumors produced by injection of T29 β-hCG and T80 β-hCG cells, respectively, revealed poorly differentiated carcinoma morphology. E–J: IHC staining of representative T80 β-hCG xenograft tumor sections, in which the intensity of brown deposition represents marker expression highlighted by blue/purple hematoxylin counterstaining, demonstrates membrane and cytoplasmic β-hCG reactivity (E), reactivity of SV40 T/t antigens indicating tumor formation by human cells (F), CD34 reactivity in recruited microvessels (G), cytoplasmic cytokeratin reactivity indicating epithelial cell lineage (H), nuclear progesterone receptor reactivity (I), and negative estrogen receptor reactivity (J).