Figure 2.

A–C: Cellular production of ROS and detoxification of H₂O₂ by N-acetyl-L-cysteine (NAC). Basal intracellular levels of O₂^•− (A), H₂O₂ (B), and NO (C) were assessed by spectrofluorometry in the various cell lines (11 Ce, 18 Ee, 17 De, 10 Cs, 18 Es, and 18 Ds). U.A., arbitrary fluorescence intensity units. *P < 0.05, **P < 0.01, and ***P < 0.001, Es versus Ce cells. ^††P < 0.01, ^†††P < 0.001, De versus Ee cells. ^‡‡P < 0.01, stromal versus epithelial cells within the same endometrial eutopic or deep infiltrating lesion. D and E: Intracellular levels of H₂O₂ (D) and proliferative rate (E) were assessed in cell lines treated with 6.4 mmol/L NAC versus untreated cells. The level in untreated cells is indicated by a horizontal dashed line. **P < 0.01, ***P < 0.001.