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Intracellular lipid content in BMSC-SMC and VSMC. Cells were treated with the 80mg/L ox-LDL for 72 hours. Cellular lipid was extracted, and free cholesterol(FC), total cholesterol(TC) were analyzed by HPLC. CE and ratio of CE/TC were calculated.
| Cells group | FC(µg/mg protein) | CE(µg/mg protein) | TC(µg/mg protein) | CE/TC(%) |
|---|---|---|---|---|
| Control | 45.9±3.2 | 5.3±2.6 | 40.1±3.5 | 13.2 |
| VSMC+ox-LDL | 86.5±4.1* | 116.6±4.5* | 196.7±9.5* | 59.3 |
| BMSC-SMC+ox-LDL | 92.1±9.4 | 167.6±8.9 | 301.3±8.5 | 55.6 |
*P<0.05 vs Control
