Figure 1.

The presence of mRNA aggregates with characteristics of stress granules (SG) is restricted to extracellular parasites. A) RNA FISH carried out with parasites grown under bradyzoite or tachyzoite conditions. (I): intracellular parasites; (E): extracellular parasites. All experiments were done with type I RH strain, except for the one indicated with (Pru) that corresponds to type II Pru strain. (Cy5): poly(A) RNA detected with oligo-dT - Cy5. DAPI staining (blue). B) Immunofluorescence assay (IFA) carried out with antibodies against Poly A Binding Protein (PABP, green), followed by RNA FISH with oligo-dT conjugated to Cy5 (Cy5, red) and DAPI staining (blue). Scale bar: 5 µm.