Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Aug 9;7(7):978–991. doi: 10.7150/ijbs.7.978

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

PMC Copyright notice

Fig 1 — Characterization of PTPBR7 ectodomain fusion proteins used for RAP in situ assays on mouse brain sections. (A) Schematic representation of mouse PTPBR7 (72 kDa) and the various BR7ecto probes (83 kDa). Signal peptide (SP), hydrophobic (HR) and transmembrane (TM) regions, kinase-interacting motif (KIM), catalytic protein tyrosine phosphatase domain (PTP), flexible Glycine linker (Gly linker), Placental alkaline phosphatase enzyme (PLAP) and the segment recognised by the α-BR7 antiserum are indicated. The seven amino acid residues (position 220, 221, 222, 223, 224, 225 and 226 in PTPBR7) that form the seven different junctions between the extracellular domain and the Glycine linker are highlighted in bold. Resulting probes are named 'BR7ecto' followed by the fusion residue number. (B) Expression and secretion of BR7ecto probes as detected on immunoblots. Conditioned culture media of HEK293T cells transiently transfected with pBG-flexilinker-based expression constructs for the various BR7ecto probes (indicated by the respective fusion residue number on the top of each lane) were subjected to SDS-PAGE and immunoblotted using rabbit polyclonal α-BR7 antiserum. The arrowhead points at the position of the BR7ecto probes. Molecular size markers (in kDa) are indicated on the left. (C) Graph reporting the alkaline phosphatase activity within the different BR7ecto probe-containing conditioned media as determined spectrophotometrically using pNPP as a substrate.