Fig 5.

Removal of CS chains does not alter PTPBR7 ectodomain binding to cerebellar white matter. BR7ecto226 probe-containing medium was incubated on Chondroitinase ABC-treated coronal sections of mouse cerebellum (B), and on non-treated control sections (A), according to the RAP in situ procedure. Low magnification images do not show overt differences in binding. Chondroitinase ABC activity was confirmed separately, exploiting GAG-specific antibodies (data not shown). Sections stained with unfused PLAP-containing medium (C) served as control. (D) Quantification of staining intensities within the white matter. Mean gray values are shown. The experiment was done in triplicate and BR7ecto226 and PLAP staining intensities were consistently found to be significantly different. Results (n=3) are presented as Mean ± S.D. (p=0.0013). CHON= chondroitinase ABC. No treat = no treatment. Bar = 1 mm.