Table 2.
Antibodies Used for Immunofluorescence Staining of Skin Sections
| Antigen | Clone | Isotype | Label | Source |
|---|---|---|---|---|
| Primary Antibodies | ||||
| CCL18 | Polyclonal | Goat IgG | Unlabeled | R&D Systems |
| CD1a | Polyclonal | Rabbit IgG | Unlabeled | Atlas Antibodies |
| CCL17 | Polyclonal | Rabbit IgG | Unlabeled | Peprotech |
| CD1a | O10 | mIgG1 | Unlabeled | DakoCytomation |
| CD1c | M241 | mIgG1, k | Biotin | Ancell |
| CD3 | F7.2.38 | mIgG1 | Unlabeled | DakoCytomation |
| CD208 | 104.G4 | mIgG1 | Unlabeled | Immunotech |
| CD11c | EP1347Y | Rabbit IgG | Unlabeled | abcam |
| CD209 | 1B10 | mIgG2a | Unlabeled | Santa Cruz |
| Ki67 | K-2 | mIgG1 | Unlabeled | Zytomed Systems |
| CD163 | 10D6 | mIgG1 | Unlabeled | Novocastra |
| Isotype Antibodies | ||||
| Mouse IgG1 | Unlabeled | BD | ||
| Goat immunoglobulin | Unlabeled | Santa Cruz | ||
| Rabbit IgG | Unlabeled | Abcam | ||
| Secondary Reagents | ||||
| Donkey anti-goat IgG (H+L) | Alexa Fluor 488 | Molecular Probes | ||
| Goat F(a,b)2 anti-rabbit IgG | Alexa Fluor 546 | Molecular Probes | ||
| Goat F(a,b)2 anti-rabbit IgG | Alexa Fluor 488 | Molecular Probes | ||
| Goat F(a,b)2 anti-mouse IgG | Alexa Fluor 546 | Invitrogen (Caltag) | ||
| Goat F(ab')2 anti-mouse IgG | Alexa Fluor 488 | Molecular Probes | ||
| Goat F(ab')2 anti-mouse IgG2a | Alexa Fluor 488 | Molecular Probes | ||
| Goat F(ab')2 anti-mouse IgG | Unlabeled | Abcam | ||
| Avidin | TXRD | Vector |