Figure 4.

The effect of siRNA knockdown of Anx A1 on U87 GBM cell growth. A: The growth curves of U87 GBM cells containing scrambled siRNAs (Anx KD-control and FPR1 KD-control), siRNAs targeting Anx A1 (Anx KD), FPR1 (FPR1 KD), or both (Double KD) were seeded (4 × 10⁴ cells) in triplicates in 12-well plate. After 24, 48, 72, and 96 hours, cell number was counted. Experiments were performed three times. *P < 0.05, compared with U87 GBM cells. ^†P < 0.05, compared with FPR1 KD-control cells. B: Anx A1 expression in tumors formed by U87 GBM cells containing Anx A1 siRNA. U87 GBM and Anx KD cells were s.c. injected into nude mice. Tumors were harvested on day 36 and sectioned, and the expression of Anx A1 was detected with immunofluorescence. Bar, 20 μm. Representative images are shown. C: The Anx A1 fluorescence intensity in transplanted tumors calculated by ImageJ.