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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Eur J Immunol. 2011 Mar 14;41(4):1086–1097. doi: 10.1002/eji.201040831

Figure 7. Silencing XBP-1 abolishes ER stress-enhanced IFN-β gene activation by polyIC.

Figure 7

DC1.2 cells were transfected with control siRNA or XBP-1 siRNA. Knock-down efficiency was confirmed by PCR (A) and immunoblotting (B). C. XBP-1 silence inhibits DC1.2 cell growth. Control or XBP-1 siRNA transduced DCs were followed by counting the viable cells at different time points. D. Following XBP-1 silencing, cells were treated with polyIC, or Tg plus polyIC, followed by qRT-PCR analysis of IFN-β (Student t-test, *p<0.005; ** p<0.001). The results are representative of three independent experiments.