Table 2. Prediction of active miRNAs based on miRNA targets derived from databases and predictions.

Datasets	Transfecting miRNA	Cell line	Time point	Primary miRNA detected
				WR Test	KS Test	Hyper. Test
Selbach et al., 2008	miR-155	Hela	8	√	√	-
	miR-155	Hela	32	√	-	√
	miR-16	Hela	8	√	√	-
	miR-16	Hela	32	√	√	√
	let-7b	Hela	8	-	-	-
	let-7b	Hela	32	√	√	-
Georges et al., 2008	miR-192	HCT116	24	√	-	√
	miR-192	HCT116	10	√	-	√
	miR-215	HCT116	10	√	-	-
	miR-215	HCT116	24	√	-	√
Baek et al., 2008	miR-124	Hela	24	√	√	-
	miR-1	Hela	24	√	√	-
	miR-181a	Hela	24	√	√	-
He et al., 2005	miR-34a	A549	24	√	√	-
	miR-34b	A549	24	√	√	-
	miR-34a	HCT116	24	√	√	√
	miR-34b	HCT116	24	√	√	-
	miR-34a	TOV21G	24	√	√	-
	miR-34b	TOV21G	24	√	√	-
	miR-34a	DLD	24	√	√	√
	miR-34b	DLD	24	√	√	-
	miR-34a	HeLa	24	√	√	√
	miR-34b	HeLa	24	√	√	-
	miR-34a	A549 p53	24	√	√	-
	miR-34b	A549 p53	24	√	√	-

The transfecting miRNAs have been detected as active in 24 out of 25 miRNA transfection experiments based on the Wilcoxon (WR) test. 8 out of 10 transfecting miRNAs in 19 out of 25 miRNA transfection experiments have been identified as active applying the Kolmogorov-Smirnov (KS) test. While 6 out of 10 transfecting miRNAs in 9 out of 25 miRNA transfection experiments have been detected applying the hypergeometric test.