Table 3. Prediction of active TFs based on the expression of their target genes.

Dataset	Transfec. miRNA	Cell line	Time point(hr)	Total	Fold chng.	FC+ stat. sig.	Statistically (stat.) sig. TFs
							WRtest	KS test	Shared KS+ WR	HGtest	Shared HG+ WR
Selbach et al., 2008	miR-155	Hela	8	27	7	0	20	6	6	0	0
	miR-155	Hela	32	30	11	3	16	13	13	6	3
	miR-16	Hela	8	20	9	0	11	2	2	1	0
	miR-16	Hela	32	34	10	0	24	17	17	0	0
	let-7b	Hela	8	27	5	1	21	9	9	5	3
	let-7b	Hela	32	25	8	1	16	9	9	5	4
Georges et al., 2008	miR-192	HCT116	24	19	2	0	17	12	12	1	0
	miR-192	HCT116	10	25	0	0	25	12	12	0	0
	miR-215	HCT116	10	20	0	0	20	10	10	0	0
	miR-215	HCT116	24	20	1	0	19	10	10	1	0
Baek et al., 2008	miR-124	Hela	24	33	4	0	29	31	28	0	0
	miR-1	Hela	24	40	2	0	38	34	34	0	0
	miR-181a	Hela	24	22	5	0	17	24	17	0	0
He et al., 2005	miR-34a	A549	24	64	0	0	64	62	61	0	0
	miR-34b	A549	24	57	0	0	57	56	52	0	0
	miR-34a	HCT116	24	65	4	1	60	58	55	0	0
	miR-34b	HCT116	24	66	3	0	63	59	58	0	0
	miR-34a	TOV21G	24	71	0	0	71	59	59	0	0
	miR-34b	TOV21G	24	64	0	0	64	62	62	0	0
	miR-34a	DLD	24	65	1	1	63	60	59	0	0
	miR-34b	DLD	24	59	3	1	55	53	51	0	0
	miR-34a	HeLa	24	64	0	0	64	62	60	0	0
	miR-34b	HeLa	24	63	0	0	63	57	56	0	0
	miR-34a	A549 p53	24	61	0	0	61	60	58	0	0
	miR-34b	A549 p53	24	59	0	0	59	60	56	0	0
Total				111	38	5	86	71	69	11	7

Overall, 86 TFs have been identified by MIRTFnet (applying the Wilcoxon test (WR)) with the used datasets. 71 TFs have been detected applying the Kolmogorov-Smirnov (KS) test and 11 TFs have been detected using the hypergeometric test. The hypergeometric test does not find any significant TF in 19 out of 25 miRNA transfection profiles.