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. 2011 Aug 17;6(8):e22566. doi: 10.1371/journal.pone.0022566

Figure 2. Validation of differentially expressed genes by real-time polymerase chain reaction.

Figure 2

We used 18 s rRNA as an internal control. Relative mRNA expression was calculated according to the 2−ΔΔT method. Data are the mean of 10 samples ± SD. Control, gene expression level in the normal colon tissue of mice in the Control group; DMH, gene expression level in the non-tumor colon tissue of mice in the DMH group; DMH+Calcium, gene expression level in the non-tumor colon tissue of mice in the DMH+Calcium group; Calcium, gene expression in the non-tumor colon tissue of mice in the Calcium group. * P value<0.05 between DMH+Calcium group and DMH group; § P value<0.05 between Calcium group and Control group.