Table 4. Expression of selection of microarray genes in embryonic tissue analysed by real-time PCR in response to iron deficiency in Rowett Hooded Lister rats (FC = fold-change).
Gene symbol | Protein | Iron | ||
P | FC | P | FC | |
Acvr2b | 0.02 | 3.35 | 0.28 | 1.65 |
C-Myc | 0.35 | 0.64 | 0.08 | 0.66 |
Ccnh | 0.26 | 1.77 | 0.44 | 1.36 |
Eif2b1 | 0.52 | 0.65 | 0.58 | 0.85 |
HNF4a | 0.07 | 0.79 | 0.42 | 0.66 |
Mcart1 | 0.34 | 0.74 | 0.82 | 0.93 |
Nufip1 | 0.00 | 0.53 | 0.30 | 0.54 |
p53 | 0.30 | 2.82 | 0.08 | 5.42 |
PMEPA1 | 0.55 | 0.87 | 0.69 | 1.15 |
Pole3 | 0.41 | 0.80 | 0.38 | 0.57 |
Prmt3 | 0.85 | 0.70 | 0.58 | 0.72 |
Psat1 | 0.22 | 0.71 | 0.10 | 0.60 |
Securin | 0.52 | 1.30 | 0.48 | 0.64 |
SP1 | 0.74 | 0.71 | 0.06 | 0.51 |
SUCLG1 | 0.87 | 0.75 | 0.40 | 0.79 |
TBX3 | 0.47 | 0.86 | 0.15 | 0.75 |
Tomm34 | 0.28 | 0.66 | 0.11 | 0.69 |
Txndc12 | 0.35 | 0.82 | 0.77 | 0.83 |
Ube2c | 0.02 | 0.55 | 0.03 | 0.45 |
The selected genes had all been shown to be significantly up- or down-regulated by whole genome microarray. n = 8 animals per group.