Table 6. Proteins identified by mass spectrometry following 2D gel electrophoresis.

Strain	Direction	Gatekeeper Protein common to low protein and iron deficient diets	Function
Wistar	Up	Protein disulfide isomerase ER60 precursorThioredoxin-like 2ARP3 actin-related protein 3 homologGuanine nucleotide-binding proteinG(i) alpha-1, chain AAlpha-fetoproteinTubulin alpha-1 chain	Protein foldingMembrane trafficking – cytoskeletonEmbryogenesis, growth regulationCytoskeletal protein
Wistar	Down	Peptidylprolyl isomerase CSUG1 protein	Protein folding26S Proteasome subunit – peptide degradation
RHL	Up	LonPhosphoserine aminotransferase26S Proteasome subunit, beta type 1	Protein degradationAmino acid biosynthesisPeptide degradation
RHL	Down	Methionine aminopeptidase 2 (Initiation factor 2-associated 67 kDa glycoprotein) (p67) (p67eIF2)Dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex)Farnesyl-pyrophosphate synthetase, testisPyruvate dehydrogenase (Lipoamide) beta1MABA f1-atpase alpha chain alpha chain A	Regulator of translationIsoprenoid biosynthesisEnergy metabolismEnergy metabolism

These proteins were significantly up- or down-regulated following prenatal protein and iron restriction within each strain of rat (RHL – Rowett Hooded Lister). n = 8 animals per group.