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. 2011 Aug 17;6(8):e23575. doi: 10.1371/journal.pone.0023575

Figure 4. Electrostatic interactions are important for binding of TMC207.

Figure 4

(A) ATP synthesis in the presence of TMC207 and increasing sodium chloride concentrations was measured for inverted membrane vesicles of Mycobacterium smegmatis (1 mg/ml). Samples were incubated at 37°C for 1 h in the presence of an ADP-regenerating system, and produced ATP was quantified spectrophotometrically by monitoring oxidation of glucose-6-phosphate with NADP+. As a control, 100 µM DCCD was added. (B) BIAcore binding studies. Purified subunit c from wild-type Mycobacterium tuberculosis was injected onto a chip with immobilized amine analog of TMC207 in the presence of 50, 150, and 300 mM NaCl at 37°C.