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Distribution of E. coli-expressed GST-opt-VP1 protein in the soluble and insoluble factions. SDS-PAGE (A) and Western-blotting (B) were performed to examine the solubility of the E. coli-expressed VP1 protein. Anti-GST tag monoclonal antibody was used to recognize the VP1 protein. Lane M, pre-stained protein marker; lane 1, soluble fraction; lane 2, insoluble fraction.
