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. 2011 Aug 17;6(8):e23725. doi: 10.1371/journal.pone.0023725

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Li et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A–B) The impact of GPR50 on TIP60-mediated GR signalling was assayed in HEK293 cells using a luciferase-based transcriptional reporter for GR (TAT3::luc)(A) and quantitative RT-PCR of a GR-responsive target gene (Fkbp5)(B). Full-length Gpr50, tGpr50, cGpr50 and/or Tip60 constructs were transfected alone or in combination as indicated.