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. 2011 Aug 17;6(8):e23725. doi: 10.1371/journal.pone.0023725

Figure 4. Endogenous TIP60 and GPR50 modulate GR signalling in GH3 cells.

Figure 4

(A–D) Contribution of endogenous GPR50 and TIP60 to GR-signalling in GH3 cells. Expression of both Gpr50 and Tip60 was confirmed in GH3 cells (A). shGpr50 or siTip60 were efficient at attenuating the expression of their respective targets as demonstrated by reduced protein accumulation following transient transfection (B) and reduced levels of endogenous mRNA transcript (C). Importantly, knockdown of either GPR50 or TIP60 in GH3 cells attenuated the induction of TAT3::luc activity in response to Dex (100 nM), although no additive effect was observed with combined knockdown of both proteins (C). The potentiation of Dex-induced TAT3::luc activity by Gpr50 over-expression was also blocked by knockdown of endogenous TIP60 using siTip60 (D). Differences in lettering reflect statistically significant differences between treatment groups (two-way ANOVA, with Bonferroni's post hoc test). Data are representative of 3 independent experiments, each performed in triplicate.