Figure 3. Inhibition of c-Myc expression in BASCs.

(A) BASCs were plated in 24-well plates at 40,000 cells per well and incubated in complete medium overnight to achieve 50% confluence. BASCs were infected with viral supernatant including control shRNA or two different c-Myc shRNAs. At day 3, day 5 and day 7 after infection, the cells were washed with PBS and images were taken under both normal light and blue light for GFP expression. (B) BASCs were suspended and stained with trypan blue at the indicated time points. Stained cells were placed in a hemocytometer and viable (unstained) cells were counted. Plotted values are the means ± SD of three replicates. (C) After several generations of puromycin selection, stable c-Myc knock down cell lines were established. c-Myc mRNA and protein expression were measured by real-time PCR and western blotting and cells were counted at the indicated time points.