Figure 2.
BVDV RNA melting curves (a) and BVDV RNA standard curve (b). (a) BVDV RNA melting curves showing 10-fold serial dilutions of standard RNA from 107 to 102 copies/ml amplified by SYBR Green RT-PCR on Bio-Rad iQ5 Multicolor Real-Time PCR Detection System. (b) BVDV RNA standard curve produced by SYBR Green RT-PCR on Bio-Rad iQ5 Multicolor Real-Time PCR Detection System using 10-fold serial dilutions of standard RNA transcribed in vitro as standard templates.