Table 1.

Analytical reproducibility of one-step SYBR Green I RT-PCR assay in the inter- and intra- assay

	Levels of cRNA (copies/ml)						TCID50
	102	103	104	105	106	107	10	10-2	10-5
The inter- assay
Ct values (mean ± S.D.)	31.63 ± 0.276	29.31 ± 0.482	25.56 ± 0.344	22.19 ± 0.286	18.55 ± 0.299	15.95 ± 0.300	24.10 ± 0.065	26.71 ± 0.570	28.87 ± 0.308
CV (%)	0.87	1.64	1.34	1.28	1.61	1.88	0.27	2.13	1.06
The intra- assay
Ct values (mean ± S.D.)	31.78 ± 0.274	29.25 ± 0.769	25.63 ± 0.327	22.30 ± 0.039	18.65 ± 0.142	16.08 ± 0.008	24.11 ± 0.046	26.71 ± 0.337	29.01 ± 0.266
CV (%)	0.86	2.63	1.27	0.17	0.76	0.05	0.19	1.26	0.91