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. 2011 Jul 12;11:292. doi: 10.1186/1471-2407-11-292

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Copyright ©2011 Su et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Double immunohistochemical staining for PR/HER2. To validate the lab staining method, commercial breast cancer tissue microarray (TMA) slides BR701 (US Biomax Inc.) were used. D1, TMA core with HER2+ and PR- staining. D2, HER2- and PR+ staining. G1, HER2+ and PR+ staining. D9, PR+ and HER2 weak-positive (borderline) staining. PR/HER2 double stains were comparable to standard single stains for HER2 and PR, although the PR signal in the double staining was somewhat weaker (original magnification: ×200).