Figure 3. PfMCA1-cd-Sc induces yeast cell death under oxidative stress.

Δyca1 yeast cells transfected with YCA1, LmjMCA-cd, PfMCA1-cd-Sc or the control vector were grown in the presence of galactose and 1 mM H₂O₂ for 30 hours with or without inhibitor (z-VAD-fmk, 20 µM). 250 cells were spread on YPG plate and cultured for 48 hours before colony-forming units were counted to estimate cell viability. Data are represented as the mean of cell viability (%)± S.D. (n = 3). a indicates a significant difference in the percentage of viability between non treated and H2O2 treated cells as determined by a Tukey's test (p<0.05). b indicates a significant difference in viability between MCA expressing lines and the vector control line in the presence of H₂O₂ as determined by a Dunnett's test (p<0.05). c indicates a significant difference in viability when z-VAD-fmk is present in comparison to conditions with H₂O₂ as determined by a Tukey's test (p<0.05).