Figure 5. PfMCA1-cd-Sc retards yeast growth under oxidative stress.

YCA1, LmjMCA-cd and PfMCA1-cd-Sc were expressed in Δyca1 cells with H₂O₂ (1 mM) and with or without inhibitor (z-VAD-fmK, 20 µM). Transfected yeasts were grown for 30 hours before yeast proliferation was determined by the MTS proliferation assay. MTS activity was measured by spectrophotometry at 492 nm every 2 hours and the growth rate was determined as described in methods. Data are represented as the mean ± S.D. (n = 3). a indicates a significant difference in the percentage of viability between non treated and H₂O₂ treated cells as determined by a Tukey's test (p<0.05). b indicates a significant difference in viability between MCA expressing lines and the vector control line in the presence of H₂O₂ as determined by a Dunnett's test (p<0.05). c indicates a significant difference in viability when z-VAD-fmk is present in comparison to conditions with H₂O₂ as determined by a Tukey's test (p<0.05).