Table 1. z-VAD-fmk decreases 3D7 P. falciparum CQ sensitivity.

	3D7			7G8
	IC50	IC90	IC95	IC50	IC90	IC95
CQ	23±11	81±28	107±24	161±55	679±260	792±275
CQ +50 µM z-VAD-fmk	206±50*	646±68*	745±70*	363±117	1095±68	1289±97
CQ +100 µM z-VAD-fmk	161±32*	560±67*	720±13*	266±96	898±205	1077±185
CQ +0.05% DMSO	16±3	58±23	84±36	172±12	488±69	582±98
CQ +0.1% DMSO	35±16	94±28	112±22	177±32	476±54	561±57

P. falciparum CQ sensitive (3D7) or resistant (7G8) clones were cultured under a graded concentration of CQ in combination with 50 µM or 100 µM z-VAD-fmk. IC₅₀, IC₉₀ and IC₉₅ were determined using the SYBR Green I fluorescence method. Use of pure DMSO at a similar percentage to that created by the addition of z-VAD-fmk acted as a control. Data are represented as the mean S.D. (n = 3). Asterisks indicate a significant increase of IC compared to IC obtained without inhibitor (* = p<0.05).